S. Shop all used microplate readers and parts from a variety of manufacturers. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Luminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. We therefore gathered salient. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. 305 reference level saturation. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. Appendix. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. 020 OD 2. 1. . SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. 1. . Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. Documentation:Plate readers that are used to detect luminescence, fluorescence and absorbance signals can vary in sensitivity (Figure 2). Devices’ StakMax® Microplate Stacker in a matter of minutes and . Selecting your dsDNA. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 00. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization. SpectraMax ABS Plus SpectraTest ABS2 v1. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. 3) At the bottom of the strip, click on "Show. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. I've got a LT4500 microplate reader and was hoping to digest the pellets in papain overnight, simply mix a measure of that sample with DMMB solution in a 96 well plate and read absorbance at 525nm. Warranty: 60. The plate reader offers a fast, simple readout of average fluorescence per well, while the cytometer gives more detailed information, including total cell number and. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Perform the following steps to install the drivers: Start > Control Panel > System. Tecan’s. Features. (30. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Driver installation. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single 1) Select the correct plate and the wells that you want to measure. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Microplate Formats: 6, 12, 24, 48, 96, 384. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Capture flash assays with ease Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax iD3 injector system withThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. Absorbance Fluorescence and fluorescence polarization Luminescence. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Fluorescence Microplate Readers 4. Instructions. The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 0) OD (0. D 4/21/06 9:53:29 AM . The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Similar settings will work for other SpectraMax readers with fluorescence detection. 1. Basic "FL Plate Reader start up protocol" (print and bring along for the. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 0 × 107) RLU 0. Brand: MOLECULAR DEVICES. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideReader Documentation Version No. Multimode Reader Comparison Chart. It is typically used to determine the concentration of nucleic acids and proteins. . the reader’s stated range. The SpectraTest absorbance test plate (Abs-1) can be used by VersaMax, SpectraMax, & M2 readers. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. 67. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. Detection modalities include absorbance (UV-Vis Abs) and. We suggest Corning 384-well plates Cat. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Using a black. Downloads. 10. patent nos. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. In general, reading fluorescence from the top is more sensitive than reading from the bottom. SpectraMax® M2/M2e Multimode Microplate Readers, with SoftMax® Pro Software, Molecular Devices. 67. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. 7°C and the bottom to 36. Reading Capabilities: cuvette or microplate. free tracer decreases, resulting in an overall increase in RFU values. Fluorescence Photometric Performance (M2) Dual monochromators: 1 nm increments: EX: 250-850 nm: EM: 250-850 nm: Bandwidth (EX, EM). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western. Molecular devices SpectraMax M5 Pdf User Manuals. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax ® Pro. The cooled PMT detector allows high sensitivity in a wide dynamic range, even in extremely low light. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 1 New Reader Support 1. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Qualify the fluorescence performance of the SpectraMax . The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. Molecular Devices SpectraMax M2 ; Molecular Devices. Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Figure 2. 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Align the two slots on the bottle holder with the two pegs on the rail. 67. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. It may be named "Pothos". The chamber. 2. 0077 OD 0. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Appendix. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. 0 out of 5 $ 14,499 $ 13,995-13%. Analyze the dataHere we report the use of the EarlyTox ™ Live/Dead Assay Kit in combination with SpectraMax ® fluorescence microplate readers (Figure 1). Molecular Devices SpectraMax M2 ; Molecular Devices. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Item Description FrontPanelOperation SinglewavelengthAbsorbance,%Transmittance,Fluorescence readingofthecuvette(ortesttube) UsingSoftMaxProSoftware ExpressdataasAbsorbance. SpectraMax ® M2 Multi-Mode Microplate Reader. In addition, the SpectraMax iD5SpectraMax Microplate Readers. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Fig 2: Enzymatic response of synthetic compounds. FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Plate reader. Optical System: High energy xenon flash lamp. Plate background OD values of disposable UV microplates vary slightly between lots. 67. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). 5 x 38. g. (a) Chemical structures of 1 (HADP) and its analogues 2–4. The SPECTRAmax® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics that permit experimental determinations at low-level detection limits. 0 software was used to handle data analysis and control each instrument. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Step 5: Click the software’s Read button. Last lab had ours for years and never had a problem with it. S. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. These lab workhorses are used in drug discovery. This reader's dual monochromators for. When the user applies a set temperature of 37°C, the top is heated to 37. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Appendix A. The modular platform allows upgrading to expand functionality. Table 1. Email. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as. 7°C and the bottom to 36. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633 (18) SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy medRxiv September 16, 2022 Harini Natarajan. 1. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Products : SpectraMax M2/M2e reader, SpectraMax. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. A . Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Danaher Inc spectramax m2 fluorescence microplate reader Spectramax M2 Fluorescence Microplate Reader, supplied by Danaher Inc, used in various techniques. 53 - 60 kg, (117-132 lb), depending on configuration. olecular saka. 306 plate air cal fail,low light. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Traditional microplate readers perform mostly biochemical assays—measuring how much light passes through or is emitted by a sample. Uses dual. If it is something different, click on the arrow to bring up a list of. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. Stage and carriage are homed and position is tested and verified. 4. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. B. Typical settings forTo maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Perkin Elmer EnVision 2104 Multilabel Plate Reader, Perkin Elmer, Envision Plate Reader. Customer Satisfaction is. Fluorescence measurements were acquired at 10 min intervals (ex: 485 nm and em: 535 nm) in a SpectraMax M2 fluorescence plate reader (Molecular Devices) operated in the 384-well microplate format. Open and turn on the laptop. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 3: The cuvette chamber. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. This opens the Settings window. 1. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. 00 USD. Protein assays. Perform the scan and note the wavelength that gives maximum emission. g. SpectraMax Paradigm Multi-Mode Safety Guide The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. I have been using the Molecular Devices SPECTRAmax GEMINI XS for the last 6 months, mostly in time-resolved fluorescence,. These robust readers have been placed in labs from Antarctica to the. Published Dec 16, 2020 | Updated Dec 16, 2020. Transcreener ® ADP. With optimized reagents, option to operate the. Data fromSpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). spr * SpectraMax i3(x) SpectraTest ABS2 v1. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. The optical systems use two scanning monochromators soUnlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Microplate Reader Shelf. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75 NTR in live cells. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. An assay plate containing a 15-point standard curve was read on the SpectraMax. . 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. For a higher . D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. Automated live cell imaging – cell counting and confluence. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. The ValitaTITER assay is based on detection of IgG Fc interactions with Protein G using fluorescence polarization (FP) technology. A solution for low volume applications. For example, microplates containing different samples can be read using the Tecan Infinite® M1000 Pro Plate Reader. ~ Bio-Tek ELx808 Microplate Reader $2,795. When connecting for the first time to either the SpectraMax L reader or StakMax system. min at 37°C. 315 can't find zero order. In the M2, M5 and Plus 384, the light. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Fluorescence Microplate Reader Comparison Chart. Download Application Note Plate reader settings for NanoOrange assay. BioTek’s Cytation™3, for instance, is a multimode plate reader that also functions as a high-speed automated digital fluorescence microscope with brightfield capability. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. 0) OD (500 to 1100) RFU (5. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). For fluorescent assays, black plates absorb light and reduce background and crosstalk. Transcreener ® ADP. 4. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Molecular Devices SpectraMax M2 Microplate Reader. S. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. Offer. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 2. Compatible automation solutions for SpectraMax Readers. 2018. Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. Factors that contribute to an instrument’s sensitivity, or limit of detection, include background noise from the detector and electronics, the type of detector that is used, and the instrument’s configuration and. Search: Submit search Search filters Search This site Search Cornell. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. When a. An approach to gain setting that I like to use is to set the gain based on the sample with the most fluorescence in order to get about 30-50% of the maximal signal before the detector saturates. Fluorescence Microplate Reader Comparison Chart. LVF Monochromator™ : Excitation and Emission. In addition, the SpectraMax iD5Popular answers (1) If you are going to read the plate from the top, you should use a solid black plate. ~ Bio-Tek ELx808 Microplate Reader $2,795. Fluorescence measurements no longer require you to adjust the gain. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. 1) Select the correct plate and the wells that you want to measure. the reader’s stated range. I understand the OD measurement in a plate reader varies with the volume of the sample measured. The calculated LOQ values were 0. 验证检测 SpectraMax 190 的工作状态。 堆板机、机械臂一体化整合 SpectraMax 190 可以在几分钟内整合到 Molecular Devices 的 StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商 合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂 进行了整合。I am interested in using a SpectraMax M2 Plate Reader for a spectrophotometric assay and am trying to collect the proper reagents and materials. SpectraMax M2 and SpectraMax M2e from Molecular Devices are multi- detection microplate readers with dual-monochromators, dual-mode cuvette ports and top- and bottom-reading capability (top-reading only on SpectraMax M2). With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. For SpectraMax L or LMax II, LMax II. 627. Cells in each image are identified by SoftMax Pro Software and cell-by-cellThe Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Main Menu Toggle. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. By employing dyes that specifically bind to nucleic acids, DNA/RNA concentrations can also be quantified using fluorescence detection. LuminescencePhotometricPerformance. You should use solid white plates for top Time- Resolved Fluorescence reads, and white plates with clear bottoms for bottom reads. The instrument will read the plate, then the relative fluorescence units (RFUs) will be displayed in the Plate section of SoftMax Pro Software. a multi-detection microplate reader with two-mode cuvette port Spectramax® m2m2e user guide SpectraMax M2e user guide cover 1 1 42106 9:53:28 AM SpectraMax M2 SpectraMax…Spectramax i3 Plate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. SpectraMax M2 plate reader (Molecular Devices) was employed to measure the absorbance at 490 nm and 680 nm. standard spectral absorbance, fluorescence, and luminescence detection. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 Instrument settings used with the SpectraMax M5 Microplate Reader. 9000-0756. 4. Tecan’s. Data from slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. • Flexible design for future expansion • User upgradable in < 2 minutes • High speed detection for up to 1536-well microplates • Excellent. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 0 out of 5 $ 9,995 $ 9,495. spectramax id5, spectramax id3 multi-mode microplate reader, spectramax id3, sof Created Date: 8/20/2020 9:11:23. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader , SpectraMax M5. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. of each sample medium was transferred to a 96-well flat-bottom plate. NOTE: This assay differs from the previous one in that the fluorescent dye ThT or ThS is included in the aggregation reaction. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Reads various kinds of 96-wells plates, and is equipped with shaking function. Product Information. Equipment Details. FAST AND ACCURATE METERTECH Microplate Reader $3,650. 0 out of 5 $ 2,999. A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. Multimode Reader Comparison Chart. 7. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. The SpectraMax Plus 384 Microplate Reader for. plate over and over again. Published Dec 16, 2020 | Updated Dec 16, 2020. The Biophysical Resource at Keck Facility offers the fluorescence plate reader as an "open access" instrument that can be used by trained users. When using the PyroWave XM Reader, excitation wavelengths of 380 nm and emission wavelengths of 440 nm are used to measure fluorescence. Linear, Orbital Slow, and Orbital Fast. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell. Figure 1. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. D 47 7. CA$14,500. 1. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Molecular Devices SpectraMax™ M2e Microplate Reader w/ Warranty. 58% for the detection module and 2. Add to Cart. 0 out of 5 $ 16,499. 00. Because. Highly selective for dsDNA over ssDNA or RNA. Then the working solution (50μL) was added to the plate for 30 min treatment without light at room temperature and then the stop solution (50μL) was added. With optimized reagents, option to operate the readers. The plate is set up for reading and images are acquired according to specified parameters. The SoftMax Pro Software validation package and IQ/OQ validation protocols include tools for FDA 21 CFR Part 11 compliance. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 0 × 103 to 2. 020 OD 2. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 9000-0756. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. For a complete list of the instruments supported by this release of theThe HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. CO 2 & O 2 control. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. For a higher degree of automation, the Automation. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. This is a result of the light being attenuated and scattered by the plastics of the well-bottom which can. Parameter EarlyTox Live/Dead EarlyTox Glutathione EarlyTox Caspase-3/7 R110 Read mode Fluorescence Fluorescence Fluorescence Read type Endpoint Endpoint Endpoint Wavelengths Lm1:. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. Wide range of applications.